EV71 Human enterovirus 71人腸道病毒71型

Product category
Viruses
Product type
Animal virus
Classification
Picornaviridae, Enterovirus
Strain designation
H
Type strain
No
Genome sequenced strain
Yes
Isolation source
Vesicular fluid from an adult female with hand, foot, and mouth disease.
Geographical isolation
China; Wuhan
Applications
Enteric disease research
Infectious disease research
Mycoplasma contamination
Not detected
Host
LLC-MK2 Derivative (CCL-7.1)
Effects
cell rounding; cell sloughing; CPE
Complete medium
EMEM (? 30-2003?) + 2% FBS (? 30-2020?)
Temperature
37°C
Atmosphere
95% Air, 5% CO2
Incubation
2 to 6 days
Recommendation for infection
Plate cells 24 hours prior to infection and infect when cultures are 70-80% confluent. Remove medium and inoculate with a small volume of virus (e.g. 1 mL per 25 cm2) diluted to provide an optimal MOI (e.g. 0.001). Adsorb 1-2 hours at 37°C in a humidified 5% CO2 atmosphere. End adsorption by adding virus growth medium.
Handling notes
Partly neutralized by high titer anti-Coxsackievirus A16 antisera and is insensitive to BrdU (40 μg/mL).
Key abbreviations
BrdU, bromodeoxyuridine
CO2, Carbon dioxide
CPE, Cytopathic effect
EMEM, Eagle's Minimum Essential Medium
FBS, Fetal bovine serum
Verification method
Whole-genome Sequencing
Deposited as
Enterovirus 71
Depositors
ZM Zheng, National Cancer Institute
Type of isolate
Human

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