pSLCAR-CD19-BBz慢病毒CAR-T表達(dá)質(zhì)粒載體

Modular 41BB-CD3z CAR backbone, For Transient Expression or Lentiviral Production
Vector typeMammalian Expression, Lentiviral
GROWTH IN BACTERIA
Bacterial Resistance(s)Ampicillin
Growth Temperature37°C
Growth Strain(s)NEB Stable
Copy numberHigh Copy
GENE/INSERT
Gene/Insert nameFMC63-41BB-3z-P2A-EGFP
Alt nameCD19(FMC63)-CAR, 41BB costim., CD3z stim domains, P2A-GFP marker
Alt nameContains: FMC63-scFV, Hinge domain, CD28-transmembrane domain, 41BB-ITD, CD3z-ITD
SpeciesH. sapiens (human)
Entrez GeneCD247 (a.k.a. CD3-ZETA, CD3H, CD3Q, CD3Z, IMD25, T3Z, TCRZ)
Entrez GeneCD28 (a.k.a. Tp44)
Entrez GeneTNFRSF9 (a.k.a. 4-1BB, CD137, CDw137, ILA)
PromoterEF1a-short
CLONING INFORMATION
Cloning methodRestriction Enzyme
5′ cloning siteBpiI (destroyed during cloning)
3′ cloning siteBpiI (destroyed during cloning)
5′ sequencing primerCGGGTTTGCCGCCA
3′ sequencing primercaggagccTGCTCCTCTTACTcc

Supplier來(lái)源:BioVector NTCC Inc.
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