vTF7-3 Vaccinia virus痘病毒毒株

Vaccinia virus
VR-2153?

Product category
Viruses
Product type
Animal virus
Classification
Poxviridae, Orthopoxvirus
Strain designation
vTF7-3 [Wr]
Type strain
No
Isolation source
Constructed in vitro.
Product format
Frozen
Specific applications
Expresses bacteriophage T[7] RNA polymerase.
Expresses bacteriophage T[7] RNA polymerase.
Characteristics
Comments
Cells which have been infected with this construct can be transfected by plasmids which contain a gene targeted for expression inserted between a T7 promoter and T7 terminator.
Expresses bacteriophage T[7] RNA polymerase.
Recombinant virus constructed in vitro.
Expresses bacteriophage T[7] RNA polymerase.
Expression of the T7 RNA polymerase is directed by the P7.5 promoter which includes early and late regulatory signals.
Constructed by transfecting vaccinia virus-infected cells with pTF703
Host
BS-C-1 (ATCC CCL-26)
Host Range: BSC-1, CV-1, HeLa, MRC-5BS-C-1 cells (ATCC CCL-26); CV-1 cells (ATCC CCL-70); HeLa cells (ATCC CCL-2); MRC-5 cells (ATCC CCL-171)
Effects
cell death; cell rounding; CPE; plaque formation; stringing
Temperature
37°C
Incubation
2 - 3 days
Handling notes
Expresses bacteriophage T(7) RNA polymerase.
Key abbreviations
BS-C-1, African green monkey kidney cells
CPE, Cytopathic effect
CV-1, African green monkey kidney cells
FBS, Fetal bovine serum
HeLa, Human cervical carcinoma cells
MRC-5, Human embryonic lung cells
NIH, National Institutes of Health

Supplier來(lái)源:BioVector NTCC Inc.
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