LU99 cell line細(xì)胞株LU-99

Comment Lung giant cell carcinoma cell line derived from human. TKG0495?(Deposited from Tohoku Univ.).
Terms and conditions There is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC.
Basic information Depositor Obinata, Masuo
 Originator Hirohashi, S. and Shimosatao, Y.
 Year of deposit 2004
 Original cell TKG0495
 Animal _human < Mammals
 Genus Homo
 Species sapiens
 Gender Male
 Age at sampling 63 years
 Tissue lung
 Disease name Giant cell carcinoma
 Classification cancer
 History Cell Resource Center for Biomedical Research, Tohoku University（TKG0495）
 Lifespan infinite
 Morphology epithelial-like
 Cellosaurus(Expasy) CVCL_3015
 deposit info lot info
 Medium Medium List
 Culture type  Adherent cells
 Medium and additives  RPMI1640 + 10% FBS
 Antibiotics  Free
 Passage method  0.25% trypsin
Culture information Passage ratio  1 : 8 split
 SC frequency  Subculture : 2 times/week
 Temperature  37 ℃
 CO2 concentration  5 %
 Freeze medium  Medium + 10% DMSO
 Freezing method  Slow freezing
 Mycoplasma  (-)
 Isozyme  LD, NP
 Chromosome mode  53-58(50) : 53(5),54(9),55(12),56(12),57(6),58(6)
 STR(human)  OK

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