Cloning vector pSP73質(zhì)粒載體-BioVector NTCC保藏中心

Standard Cloning Vector That Also Transcribes RNA In Vitro

• Versatile vector that can be used for standard cloning and in vitro transcription

• SP6 and T7 RNA polymerase promoters flank the multiple cloning region

• Multiple cloning site provides a convenient selection of restriction sites for cloning

The pSP73 Vector offers a wide range of restriction sites, providing greater versatility in cloning and transcription of RNA in vitro. The pSP73 Vector contains the SP6 and T7 RNA polymerase promoters and a unique multiple cloning region, which includes restriction sites for BglII, EcoRV, ClaI, EcoRI, SacI, KpnI, SmaI, BamHI, XbaI, SalI, AccI, PstI, SphI, HindIII, PvuII and XhoI. The pSP72 and pSP73 Vectors are essentially identical except for the orientation of the multiple cloning region.

Reference

1. Krieg, P.A. and Melton, D.A. (1987) Meth. Enzymol.155, 397–415.