pShuttle-IRES-hrGFP-1腺病毒表達(dá)載體質(zhì)粒圖譜序列抗性價(jià)格報(bào)價(jià)Biovector NTCC Inc.
- 價(jià) 格:¥1920
- 貨 號(hào):BiovectorShuttle-IRES-hrGFP-1
- 產(chǎn) 地:北京
- BioVector NTCC典型培養(yǎng)物保藏中心
- 聯(lián)系人:Dr.Xu, Biovector NTCC Inc.
電話:400-800-2947 工作微信:1843439339 (QQ同號(hào))
手機(jī):18901268599
地址:北京
- 已注冊(cè)
載體基本信息
| 出品公司: | Agilent |
|---|---|
| 載體名稱: | pShuttle-IRES-hrGFP-1 |
| 質(zhì)粒類型: | 腺病毒表達(dá)載體,哺乳動(dòng)物細(xì)胞表達(dá)載體 |
| 高拷貝/低拷貝: | 低拷貝 |
| 啟動(dòng)子: | CMV |
| 克隆方法: | 多克隆位點(diǎn),限制性內(nèi)切酶 |
| 載體大小: | 8.9 kb |
| 5' 測(cè)序引物及序列: | pShuttle-IRES-hrGFP-1-F: CTCACGGGGATTTCCAAGTC |
| 3' 測(cè)序引物及序列: | pShuttle-IRES-hrGFP-1-R: ATGCAGTCGTCGAGGAATTG |
| 載體標(biāo)簽: | -- |
| 載體抗性: | 卡那 |
| 備注: | 載體內(nèi)包含有IRES序列和hrGFP序列。 |
| 產(chǎn)品目錄號(hào): | 240081 |
| 穩(wěn)定性: | / |
| 組成型: | -- |
| 病毒/非病毒: | 腺病毒 |
載體質(zhì)粒圖譜和多克隆位點(diǎn)信息
載體簡(jiǎn)介
Two additional shuttle vectors, pShuttle-IRES-hrGFP-1 (Catalog #240081) and pShuttle-IRES-hrGFP-2 (Catalog #240082) are available separately. Both of these vectors contain the CMV promoter and a dicistronic expression cassette in which the multiple cloning site (MCS) is followed by the EMCV-IRES, which directs translation of a humanized recombinant green fluorescent protein (hrGFP) from a novel marine organism as a second open reading frame. This design allows the expression of the gene of interest (up to 5.2 kb) to be monitored at the single-cell level due to expression of the hrGFP on the same transcript. The gene of interest may be fused to three contiguous copies of either the FLAG? epitope (pShuttle-IRES-hrGFP-1) or the HA epitope (pShuttle-IRES-hrGFP-2).
載體序列
載體質(zhì)粒圖譜pdf版和相關(guān)資料下載
載體應(yīng)用舉例
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