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首頁(yè) ? pShuttle-IRES-hrGFP-1腺病毒表達(dá)載體質(zhì)粒圖譜序列抗性價(jià)格報(bào)價(jià)Biovector NTCC Inc.

pShuttle-IRES-hrGFP-1腺病毒表達(dá)載體質(zhì)粒圖譜序列抗性價(jià)格報(bào)價(jià)Biovector NTCC Inc.

  • 價(jià)  格:¥1920
  • 貨  號(hào):BiovectorShuttle-IRES-hrGFP-1
  • 產(chǎn)  地:北京
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載體基本信息

出品公司:Agilent
載體名稱:pShuttle-IRES-hrGFP-1
質(zhì)粒類型:腺病毒表達(dá)載體,哺乳動(dòng)物細(xì)胞表達(dá)載體
高拷貝/低拷貝:低拷貝
啟動(dòng)子:CMV
克隆方法:多克隆位點(diǎn),限制性內(nèi)切酶
載體大小:8.9 kb
5' 測(cè)序引物及序列:pShuttle-IRES-hrGFP-1-F: CTCACGGGGATTTCCAAGTC
3' 測(cè)序引物及序列:pShuttle-IRES-hrGFP-1-R: ATGCAGTCGTCGAGGAATTG
載體標(biāo)簽:--
載體抗性:卡那
備注:載體內(nèi)包含有IRES序列和hrGFP序列。
產(chǎn)品目錄號(hào):240081
穩(wěn)定性:/
組成型:--
病毒/非病毒:腺病毒

載體質(zhì)粒圖譜和多克隆位點(diǎn)信息

pShuttle-IRES-hrGFP-1載體圖譜


pShuttle-IRES-hrGFP-1多克隆位點(diǎn)
pShuttle-IRES-hrGFP-1載體特征

載體簡(jiǎn)介

Two additional shuttle vectors, pShuttle-IRES-hrGFP-1 (Catalog #240081) and pShuttle-IRES-hrGFP-2 (Catalog #240082) are available separately. Both of these vectors contain the CMV promoter and a dicistronic expression cassette in which the multiple cloning site (MCS) is followed by the EMCV-IRES, which directs translation of a humanized recombinant green fluorescent protein (hrGFP) from a novel marine organism as a second open reading frame. This design allows the expression of the gene of interest (up to 5.2 kb) to be monitored at the single-cell level due to expression of the hrGFP on the same transcript. The gene of interest may be fused to three contiguous copies of either the FLAG? epitope (pShuttle-IRES-hrGFP-1) or the HA epitope (pShuttle-IRES-hrGFP-2).

載體序列

載體質(zhì)粒圖譜pdf版和相關(guān)資料下載


載體應(yīng)用舉例




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