T4 Lysozyme WT* plasmid

Vector backbone pHS1403

Backbone size w/o insert (bp)5000

Vector typeBacterial Expression

GROWTH IN BACTERIA

Bacterial Resistance(s)Ampicillin

Growth Temperature37°C

Growth Strain(s)DH5alpha

Copy numberHigh Copy

GENE/INSERT

Gene/Insert nameT4 Lysozyme

SpeciesBacteriophage T4

Insert Size (bp)600

MutationNo cysteines: C54T, C97A

GenBank IDNP_049736

Entrez Genee (a.k.a. T4p123)

CLONING INFORMATION

Cloning methodRestriction Enzyme

5′ cloning siteBamHI (not destroyed)

3′ cloning siteHindIII (not destroyed)

5′ sequencing primerna

3′ sequencing primerGGCGTATCACGAGGCCC