The ExpiCHO Expression System is designed to allow high-density transfection of suspension ExpiCHO-S cells in a defined, serum-free medium. The system includes ExpiCHO-S cells that have been adapted to serum-free, high-density suspension culture in ExpiCHO Expression Medium. Transfection and expression experiments may be performed directly in ExpiCHO Expression Medium without the need for media change. The ExpiCHOExpression System Kit provides cells, culture medium, and reagents to transfect 1 liter of cell culture, as described below. Except for the ExpiCHO-S cells, all of the components of the ExpiCHO Expression System are animal origin-free. ExpiCHO-S Cells The ExpiCHO-S cell line is a clonal derivative of the CHO-S cell line. The ExpiCHO-S cells are adapted to high-density suspension culture in ExpiCHO? Expression Medium. Frozen cells are supplied in, and may be thawed directly into, ExpiCHO Expression Medium. The ExpiCHO-S cell line exhibits the following characteristics: ? Derived from the same parental lineage as the CHO-S cell line ? Rapid recovery post-thaw ? Adapted to high density, serum-free, suspension growth in ExpiCHO Expression Medium ? Doubling time of approximately 17 hours ? Reaches maximum cell densities of approximately 20 × 106 cells/mL in shake flask culture ? High protein expression ? Stable transient expression over passages The ExpiCHO-S? cells are also available separately; see page 20 for ordering information. ExpiCHO Expression Medium ExpiCHO? Expression Medium is a chemically-defined medium developed specifically for the high-density culture and transfection of ExpiCHO-S cells in suspension. ExpiCHO Expression Medium requires no additional supplementation. ExpiCHO Expression Medium exhibits the following features: ? Formulated as an optimized, chemically-defined, serum-free, protein-free, animal origin-free medium to support the high-density culture and transfection of ExpiCHO-S cells in suspension. ? Supplemented with GlutaMAX-I. ? Does not interfere with nor reduce the activity of ExpiFectamine CHO Reagent. ? Designed for scalable transient transfection and protein expression.

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