GV3101/pSoup strain根癌農(nóng)桿菌菌株/感受態(tài)細胞

The genotype of GV3101 (pSoup) Competent Cell is C58 (rifR) Ti pMP90 (pTiC58DT-DNA) (gentR) Nopaline(pSoup-tetR). The background of the GV3101 (pSoup) strain is C58 type, and the nuclear gene of GV3101 contains a screening tag - rifampicin resistance gene (rif). In order to facilitate the transformation, the GV3101 strain carries a nopaline-based Ti plasmid pMP90 (pTiC58DT-DNA) without self-transport function, which contains the vir gene. The vir gene is an essential component for the insertion of T-DNA into the plant genome, and the T-DNA transfer function of the pMP90 (pTiC58DT-DNA) plasmid itself is disrupted, but the foreign binary vector T-DNA can be transferred smoothly. Since the pMP90 (pTiC58DT-DNA) plasmid contains the screening tag: gent, the GV3101 strain has gentamicin resistance. Transferring the pSoup plasmid into the GV3101 strain is the GV3101 (pSoup) strain, which helps the pGreen, 62SK, pGs2 series plasmids to replicate in Agrobacterium and helps the GV3101 (pSoup) strain to have tetracycline (tet) resistance.
Applications
GV3101 (pSoup) Competent Cell is suitable for transgenic operations of Arabidopsis, tobacco, corn, potatoes and other plants.


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