pTTB2枯草芽孢桿菌食品級(jí)表達(dá)載體質(zhì)粒Bacillus_subtilis_Food_Grade expression vector

The BacillusFood Grade Selection Systemprovidesthe following features:-Stable high-or low-level expression without addition of any antibiotics-All DNA contained in the final expression system is derived from B. subtilis-No endotoxins are produced-No inclusion bodies are formed-Protease-deficient strain for producing secretory enzymes is availableThe FoodGradeSelection SystemThe BacillusFoodGrade Selection Systemis based on the interplay of anendogenous BacillustoxinEndoAand its antitoxinEndoB.EndoA (encoded by ydcE) is an endoribonuclease that specifically cleaves mRNA at a five Base U?ACAU sequence(Pellegrini et al.,2005, Park et al.,2011). During normal growth conditions EndoA  is  inactivated  by  forming  a  heterohexameric complex  with  its  cognate antitoxin EndoB (encoded by ydcD; Simanshu et al., 2013).Since the antitoxin is relatively unstable, it is essential for the cell to continuously produce sufficient amounts of EndoB to inactivate  the morestable toxin.These  characteristics  are  utilized  for the here  describedselection system.

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