pAdTrack腺病毒表達(dá)載體質(zhì)粒圖譜序列抗性價(jià)格報(bào)價(jià)Biovector NTCC Inc.
- 價(jià) 格：￥1920
- 貨 號：BiovectorAdTrack
- 產(chǎn) 地：北京
- BioVector NTCC典型培養(yǎng)物保藏中心
- 聯(lián)系人：Dr.Xu, Biovector NTCC Inc.
電話：400-800-2947 工作微信:1843439339 (QQ同號)
手機(jī)：18901268599
地址：北京
- 已注冊
載體基本信息
| 出品公司: | Agilent |
|---|---|
| 載體名稱: | pAdTrack |
| 質(zhì)粒類型: | 哺乳動(dòng)物細(xì)胞腺病毒表達(dá)載體 |
| 高拷貝/低拷貝: | 低拷貝 |
| 啟動(dòng)子: | -- |
| 克隆方法: | 多克隆位點(diǎn)，限制性內(nèi)切酶 |
| 載體大小: | 8175 bp |
| 5' 測序引物及序列: | / |
| 3' 測序引物及序列: | / |
| 載體標(biāo)簽: | EGFP |
| 載體抗性: | 卡那 |
| 備注: | pAdTrack載體用來轉(zhuǎn)基因腺病毒細(xì)胞表達(dá)，載體擁有一個(gè)EGFP綠色熒光蛋白跟蹤基因表達(dá)結(jié)果，和pAdeasy1載體共同使用時(shí)最大能夠插入5.9kb，pAdeasy2載體共同使用時(shí)能夠最大插入8.6kb片段。具體使用方法可以參考pAdeasy載體系統(tǒng)。 |
| 產(chǎn)品目錄號: | 240005 |
| 穩(wěn)定性: | / |
| 組成型: | -- |
| 病毒/非病毒: | 腺病毒 |
載體質(zhì)粒圖譜和多克隆位點(diǎn)信息
載體簡介
載體序列
LOCUS pAdTrack 8175 bp DNA circular SYN
DEFINITION pAdTrack
ACCESSION
KEYWORDS
SOURCE
ORGANISM other sequences; artificial sequences; vectors.
COMMENT This file is created by Vector NTI
http://www.nedfriskphoto.com/
COMMENT VNTAUTHORNAME|biovector.net|
FEATURES Location/Qualifiers
source 1..8175
/organism="pAdTrack"
/mol_type="other DNA"
misc_feature 197..345
/label="Encap"
misc_feature complement(374..393)
/label="EBV_rev_primer"
CDS 575..1354
/label="ORF frame 2"
CDS complement(593..1339)
/label="ORF frame 3"
gene complement(623..1336)
/label="EGFP"
/gene="EGFP"
misc_feature complement(665..686)
/label="EGFP_C_primer"
misc_feature complement(1127..1156)
/label="Y66 (EGFP)"
misc_feature 1273..1294
/label="EGFP_N_primer"
misc_feature complement(1413..1433)
/label="CMV_fwd_primer"
CDS complement(2563..3921)
/label="ORF frame 1"
CDS complement(3669..4190)
/label="ORF frame 2"
misc_feature complement(5147..5242)
/label="LITR"
misc_feature 5147..5242
/label="RITR"
rep_origin complement(5487..6106)
/label="pBR322_origin"
gene complement(6261..6560)
/label="Ampicillin (299 - 0)"
/gene="Ampicillin (299 - 0)"
promoter 6765..6814
/label="NEOKAN_promoter"
CDS 6903..7697
/label="ORF frame 3"
gene 6906..7694
/label="NeoR/KanR"
/gene="NeoR/KanR"
ORIGIN
1 NNNTTAATTA ANNNTCCCTT CCAGCTCTCT GCCCCTTTTG GATTGAAGCC AATATGATAA
61 TGAGGGGGTG GAGTTTGTGA CGTGGCGCGG GGCGTGGGAA CGGGGCGGGT GACGTAGTAG
121 TGTGGCGGAA GTGTGATGTT GCAAGTGTGG CGGAACACAT GTAAGCGACG GATGTGGCAA
181 AAGTGACGTT TTTGGTGTGC GCCGGTGTAC ACAGGAAGTG ACAATTTTCG CGCGGTTTTA
241 GGCGGATGTT GTAGTAAATT TGGGCGTAAC CGAGTAAGAT TTGGCCATTT TCGCGGGAAA
301 ACTGAATAAG AGGAAGTGAA ATCTGAATAA TTTTGTGTTA CTCATAGCGC GTAANNNCGC
361 GTTAAGATAC ATTGATGAGT TTGGACAAAC CACAACTAGA ATGCAGTGAA AAAAATGCTT
421 TATTTGTGAA ATTTGTGATG CTATTGCTTT ATTTGTAACC ATTATAAGCT GCAATAAACA
481 AGTTAACAAC AACAATTGCA TTCATTTTAT GTTTCAGGTT CAGGGGGAGG TGTGGGAGGT
541 TTTTTAAAGC AAGTAAAACC TCTACAAATG TGGTATGGCT GATTATGATC AGTTATCTAG
601 ATCCGGTGGA TCTGAGTCCG GACTTGTACA GCTCGTCCAT GCCGAGAGTG ATCCCGGCGG
661 CGGTCACGAA CTCCAGCAGG ACCATGTGAT CGCGCTTCTC GTTGGGGTCT TTGCTCAGGG
721 CGGACTGGGT GCTCAGGTAG TGGTTGTCGG GCAGCAGCAC GGGGCCGTCG CCGATGGGGG
781 TGTTCTGCTG GTAGTGGTCG GCGAGCTGCA CGCTGCCGTC CTCGATGTTG TGGCGGATCT
841 TGAAGTTCAC CTTGATGCCG TTCTTCTGCT TGTCGGCCAT GATATAGACG TTGTGGCTGT
901 TGTAGTTGTA CTCCAGCTTG TGCCCCAGGA TGTTGCCGTC CTCCTTGAAG TCGATGCCCT
961 TCAGCTCGAT GCGGTTCACC AGGGTGTCGC CCTCGAACTT CACCTCGGCG CGGGTCTTGT
1021 AGTTGCCGTC GTCCTTGAAG AAGATGGTGC GCTCCTGGAC GTAGCCTTCG GGCATGGCGG
1081 ACTTGAAGAA GTCGTGCTGC TTCATGTGGT CGGGGTAGCG GCTGAAGCAC TGCACGCCGT
1141 AGGTCAGGGT GGTCACGAGG GTGGGCCAGG GCACGGGCAG CTTGCCGGTG GTGCAGATGA
1201 ACTTCAGGGT CAGCTTGCCG TAGGTGGCAT CGCCCTCGCC CTCGCCGGAC ACGCTGAACT
1261 TGTGGCCGTT TACGTCGCCG TCCAGCTCGA CCAGGATGGG CACCACCCCG GTGAACAGCT
1321 CCTCGCCCTT GCTCACCATG GTGGCGACCG GTAGCGCTAG CGGATCTGAC GGTTCACTAA
1381 ACCAGCTCTG CTTATATAGA CCTCCCACCG TACACGCCTA CCGCCCATTT GCGTCAATGG
1441 GGCGGAGTTG TTACGACATT TTGGAAAGTC CCGTTGATTT TGGTGCCAAA ACAAACTCCC
1501 ATTGACGTCA ATGGGGTGGA GACTTGGAAA TCCCCGTGAG TCAAACCGCT ATCCACGCCC
1561 ATTGATGTAC TGCCAAAACC GCATCACCAT GGTAATAGCG ATGACTAATA CGTAGATGTA
1621 CTGCCAAGTA GGAAAGTCCC ATAAGGTCAT GTACTGGGCA TAATGCCAGG CGGGCCATTT
1681 ACCGTCATTG ACGTCAATAG GGGGCGTACT TGGCATATGA TACACTTGAT GTACTGCCAA
1741 GTGGGCAGTT TACCGTAAAT ACTCCACCCA TTGACGTCAA TGGAAAGTCC CTATTGGCGT
1801 TACTATGGGA ACATACGTCA TTATTGACGT CAATGGGCGG GGGTCGTTGG GCGGTCAGCC
1861 AGGCGGGCCA TTTACCGTAA GTTATGTAAC GCGGAACTCC ATATATGGGC TATGAACTAA
1921 TGACCCCGTA ATTGATTACT ATTANNNCTA GCAGATCTGG TACCGTCGAC GCGGCCGCGA
1981 TATCCTCGAG AAGCTTTCTA GAGNNNTAAG GGTGGGAAAG AATATATAAG GTGGGGGTCT
2041 TATGTAGTTT TGTATCTGTT TTGCAGCAGC CGCCGCCGCC ATGAGCACCA ACTCGTTTGA
2101 TGGAAGCATT GTGAGCTCAT ATTTGACAAC GCGCATGCCC CCATGGGCCG GGGTGCGTCA
2161 GAATGTGATG GGCTCCAGCA TTGATGGTCG CCCCGTCCTG CCCGCAAACT CTACTACCTT
2221 GACCTACGAG ACCGTGTCTG GAACGCCGTT GGAGACTGCA GCCTCCGCCG CCGCTTCAGC
2281 CGCTGCAGCC ACCGCCCGCG GGATTGTGAC TGACTTTGCT TTCCTGAGCC CGCTTGCAAG
2341 CAGTGCAGCT TCCCGTTCAT CCGCCCGCGA TGACAAGTTG ACGGCTCTTT TGGCACAATT
2401 GGATTCTTTG ACCCGGGAAC TTAATGTCGT TTCTCAGCAG CTGTTGGATC TGCGCCAGCA
2461 GGTTTCTGCC CTGAAGGCTT CCTCCCCTCC CAATGCGGTT TAAAACATAA ATAAAAAACC
2521 AGACTCTGTT TGGATTTGGA TCAAGCAAGT GTCTTGCTGT CTTTATTTAG GGGTTTTGCG
2581 CGCGCGGTAG GCCCGGGACC AGCGGTCTCG GTCGTTGAGG GTCCTGTGTA TTTTTTCCAG
2641 GACGTGGTAA AGGTGACTCT GGATGTTCAG ATACATGGGC ATAAGCCCGT CTCTGGGGTG
2701 GAGGTAGCAC CACTGCAGAG CTTCATGCTG CGGGGTGGTG TTGTAGATGA TCCAGTCGTA
2761 GCAGGAGCGC TGGGCGTGGT GCCTAAAAAT GTCTTTCAGT AGCAAGCTGA TTGCCAGGGG
2821 CAGGCCCTTG GTGTAAGTGT TTACAAAGCG GTTAAGCTGG GATGGGTGCA TACGTGGGGA
2881 TATGAGATGC ATCTTGGACT GTATTTTTAG GTTGGCTATG TTCCCAGCCA TATCCCTCCG
2941 GGGATTCATG TTGTGCAGAA CCACCAGCAC AGTGTATCCG GTGCACTTGG GAAATTTGTC
3001 ATGTAGCTTA GAAGGAAATG CGTGGAAGAA CTTGGAGACG CCCTTGTGAC CTCCAAGATT
3061 TTCCATGCAT TCGTCCATAA TGATGGCAAT GGGCCCACGG GCGGCGGCCT GGGCGAAGAT
3121 ATTTCTGGGA TCACTAACGT CATAGTTGTG TTCCAGGATG AGATCGTCAT AGGCCATTTT
3181 TACAAAGCGC GGGCGGAGGG TGCCAGACTG CGGTATAATG GTTCCATCCG GCCCAGGGGC
3241 GTAGTTACCC TCACAGATTT GCATTTCCCA CGCTTTGAGT TCAGATGGGG GGATCATGTC
3301 TACCTGCGGG GCGATGAAGA AAACGGTTTC CGGGGTAGGG GAGATCAGCT GGGAAGAAAG
3361 CAGGTTCCTG AGCAGCTGCG ACTTACCGCA GCCGGTGGGC CCGTAAATCA CACCTATTAC
3421 CGGGTGCAAC TGGTAGTTAA GAGAGCTGCA GCTGCCGTCA TCCCTGAGCA GGGGGGCCAC
3481 TTCGTTAAGC ATGTCCCTGA CTCGCATGTT TTCCCTGACC AAATCCGCCA GAAGGCGCTC
3541 GCCGCCCAGC GATAGCAGTT CTTGCAAGGA AGCAAAGTTT TTCAACGGTT TGAGACCGTC
3601 CGCCGTAGGC ATGCTTTTGA GCGTTTGACC AAGCAGTTCC AGGCGGTCCC ACAGCTCGGT
3661 CACCTGCTCT ACGGCATCTC GATCCAGCAT ATCTCCTCGT TTCGCGGGTT GGGGCGGCTT
3721 TCGCTGTACG GCAGTAGTCG GTGCTCGTCC AGACGGGCCA GGGTCATGTC TTTCCACGGG
3781 CGCAGGGTCC TCGTCAGCGT AGTCTGGGTC ACGGTGAAGG GGTGCGCTCC GGGCTGCGCG
3841 CTGGCCAGGG TGCGCTTGAG GCTGGTCCTG CTGGTGCTGA AGCGCTGCCG GTCTTCGCCC
3901 TGCGCGTCGG CCAGGTAGCA TTTGACCATG GTGTCATAGT CCAGCCCCTC CGCGGCGTGG
3961 CCCTTGGCGC GCAGCTTGCC CTTGGAGGAG GCGCCGCACG AGGGGCAGTG CAGACTTTTG
4021 AGGGCGTAGA GCTTGGGCGC GAGAAATACC GATTCCGGGG AGTAGGCATC CGCGCCGCAG
4081 GCCCCGCAGA CGGTCTCGCA TTCCACGAGC CAGGTGAGCT CTGGCCGTTC GGGGTCAAAA
4141 ACCAGGTTTC CCCCATGCTT TTTGATGCGT TTCTTACCTC TGGTTTCCAT GAGCCGGTGT
4201 CCACGCTCGG TGACGAAAAG GCTGTCCGTG TCCCCGTATA CAGACTNNNG TTTAAACGAA
4261 TTCNNNTATA AAATGCAAGG TGCTGCTCAA AAAATCAGGC AAAGCCTCGC GCAAAAAAGA
4321 AAGCACATCG TAGTCATGCT CATGCAGATA AAGGCAGGTA AGCTCCGGAA CCACCACAGA
4381 AAAAGACACC ATTTTTCTCT CAAACATGTC TGCGGGTTTC TGCATAAACA CAAAATAAAA
4441 TAACAAAAAA ACATTTAAAC ATTAGAAGCC TGTCTTACAA CAGGAAAAAC AACCCTTATA
4501 AGCATAAGAC GGACTACGGC CATGCCGGCG TGACCGTAAA AAAACTGGTC ACCGTGATTA
4561 AAAAGCACCA CCGACAGCTC CTCGGTCATG TCCGGAGTCA TAATGTAAGA CTCGGTAAAC
4621 ACATCAGGTT GATTCATCGG TCAGTGCTAA AAAGCGACCG AAATAGCCCG GGGGAATACA
4681 TACCCGCAGG CGTAGAGACA ACATTACAGC CCCCATAGGA GGTATAACAA AATTAATAGG
4741 AGAGAAAAAC ACATAAACAC CTGAAAAACC CTCCTGCCTA GGCAAAATAG CACCCTCCCG
4801 CTCCAGAACA ACATACAGCG CTTCACAGCG GCAGCCTAAC AGTCAGCCTT ACCAGTAAAA
4861 AAGAAAACCT ATTAAAAAAA CACCACTCGA CACGGCACCA GCTCAATCAG TCACAGTGTA
4921 AAAAAGGGCC AAGTGCAGAG CGAGTATATA TAGGACTAAA AAATGACGTA ACGGTTAAAG
4981 TCCACAAAAA ACACCCAGAA AACCGCACGC GAACCTACGC CCAGAAACGA AAGCCAAAAA
5041 ACCCACAACT TCCTCAAATC GTCACTTCCG TTTTCCCACG TTACGTAACT TCCCATTTTA
5101 AGAAAACTAC AATTCCCAAC ACATACAAGT TACTCCGCCC TAAAACCTAC GTCACCCGCC
5161 CCGTTCCCAC GCCCCGCGCC ACGTCACAAA CTCCACCCCC TCATTATCAT ATTGGCTTCA
5221 ATCCAAAATA AGGTATATTA TTGATGATNN NTTAATTAAG GATCCNNNCG GTGTGAAATA
5281 CCGCACAGAT GCGTAAGGAG AAAATACCGC ATCAGGCGCT CTTCCGCTTC CTCGCTCACT
5341 GACTCGCTGC GCTCGGTCGT TCGGCTGCGG CGAGCGGTAT CAGCTCACTC AAAGGCGGTA
5401 ATACGGTTAT CCACAGAATC AGGGGATAAC GCAGGAAAGA ACATGTGAGC AAAAGGCCAG
5461 CAAAAGGCCA GGAACCGTAA AAAGGCCGCG TTGCTGGCGT TTTTCCATAG GCTCCGCCCC
5521 CCTGACGAGC ATCACAAAAA TCGACGCTCA AGTCAGAGGT GGCGAAACCC GACAGGACTA
5581 TAAAGATACC AGGCGTTTCC CCCTGGAAGC TCCCTCGTGC GCTCTCCTGT TCCGACCCTG
5641 CCGCTTACCG GATACCTGTC CGCCTTTCTC CCTTCGGGAA GCGTGGCGCT TTCTCATAGC
5701 TCACGCTGTA GGTATCTCAG TTCGGTGTAG GTCGTTCGCT CCAAGCTGGG CTGTGTGCAC
5761 GAACCCCCCG TTCAGCCCGA CCGCTGCGCC TTATCCGGTA ACTATCGTCT TGAGTCCAAC
5821 CCGGTAAGAC ACGACTTATC GCCACTGGCA GCAGCCACTG GTAACAGGAT TAGCAGAGCG
5881 AGGTATGTAG GCGGTGCTAC AGAGTTCTTG AAGTGGTGGC CTAACTACGG CTACACTAGA
5941 AGGACAGTAT TTGGTATCTG CGCTCTGCTG AAGCCAGTTA CCTTCGGAAA AAGAGTTGGT
6001 AGCTCTTGAT CCGGCAAACA AACCACCGCT GGTAGCGGTG GTTTTTTTGT TTGCAAGCAG
6061 CAGATTACGC GCAGAAAAAA AGGATCTCAA GAAGATCCTT TGATCTTTTC TACGGGGTCT
6121 GACGCTCAGT GGAACGAAAA CTCACGTTAA GGGATTTTGG TCATGAGATT ATCAAAAAGG
6181 ATCTTCACCT AGATCCTTTT AAATTAAAAA TGAAGTTTTA AATCAATCTA AAGTATATAT
6241 GAGTAAACTT GGTCTGACAG TTACCAATGC TTAATCAGTG AGGCACCTAT CTCAGCGATC
6301 TGTCTATTTC GTTCATCCAT AGTTGCCTGA CTCCCCGTCG TGTAGATAAC TACGATACGG
6361 GAGGGCTTAC CATCTGGCCC CAGTGCTGCA ATGATACCGC GAGACCCACG CTCACCGGCT
6421 CCAGATTTAT CAGCAATAAA CCAGCCAGCC GGAAGGGCCG AGCGCAGAAG TGGTCCTGCA
6481 ACTTTATCCG CCTCCATCCA GTCTATTAAT TGTTGCCGGG AAGCTAGAGT AAGTAGTTCG
6541 CCAGTTAATA GTTTGCGCAA CGTTGTTGNN NNNNAAAAAG GATCTTCACC TAGATCCTTT
6601 TCACGTAGAA AGCCAGTCCG CAGAAACGGT GCTGACCCCG GATGAATGTC AGCTACTGGG
6661 CTATCTGGAC AAGGGAAAAC GCAAGCGCAA AGAGAAAGCA GGTAGCTTGC AGTGGGCTTA
6721 CATGGCGATA GCTAGACTGG GCGGTTTTAT GGACAGCAAG CGAACCGGAA TTGCCAGCTG
6781 GGGCGCCCTC TGGTAAGGTT GGGAAGCCCT GCAAAGTAAA CTGGATGGCT TTCTCGCCGC
6841 CAAGGATCTG ATGGCGCAGG GGATCAAGCT CTGATCAAGA GACAGGATGA GGATCGTTTC
6901 GCATGATTGA ACAAGATGGA TTGCACGCAG GTTCTCCGGC CGCTTGGGTG GAGAGGCTAT
6961 TCGGCTATGA CTGGGCACAA CAGACAATCG GCTGCTCTGA TGCCGCCGTG TTCCGGCTGT
7021 CAGCGCAGGG GCGCCCGGTT CTTTTTGTCA AGACCGACCT GTCCGGTGCC CTGAATGAAC
7081 TGCAAGACGA GGCAGCGCGG CTATCGTGGC TGGCCACGAC GGGCGTTCCT TGCGCAGCTG
7141 TGCTCGACGT TGTCACTGAA GCGGGAAGGG ACTGGCTGCT ATTGGGCGAA GTGCCGGGGC
7201 AGGATCTCCT GTCATCTCAC CTTGCTCCTG CCGAGAAAGT ATCCATCATG GCTGATGCAA
7261 TGCGGCGGCT GCATACGCTT GATCCGGCTA CCTGCCCATT CGACCACCAA GCGAAACATC
7321 GCATCGAGCG AGCACGTACT CGGATGGAAG CCGGTCTTGT CGATCAGGAT GATCTGGACG
7381 AAGAGCATCA GGGGCTCGCG CCAGCCGAAC TGTTCGCCAG GCTCAAGGCG AGCATGCCCG
7441 ACGGCGAGGA TCTCGTCGTG ACCCATGGCG ATGCCTGCTT GCCGAATATC ATGGTGGAAA
7501 ATGGCCGCTT TTCTGGATTC ATCGACTGTG GCCGGCTGGG TGTGGCGGAC CGCTATCAGG
7561 ACATAGCGTT GGCTACCCGT GATATTGCTG AAGAGCTTGG CGGCGAATGG GCTGACCGCT
7621 TCCTCGTGCT TTACGGTATC GCCGCTCCCG ATTCGCAGCG CATCGCCTTC TATCGCCTTC
7681 TTGACGAGTT CTTCTGAATT TTGTTAAAAT TTTTGTTAAA TCAGCTCATT TTTTAACCAA
7741 TAGGCCGAAA TCGGCAACAT CCCTTATAAA TCAAAAGAAT AGACCGCGAT AGGGTTGAGT
7801 GTTGTTCCAG TTTGGAACAA GAGTCCACTA TTAAAGAACG TGGACTCCAA CGTCAAAGGG
7861 CGAAAAACCG TCTATCAGGG CGATGGCCCA CTACGTGAAC CATCACCCAA ATCAAGTTTT
7921 TTGCGGTCGA GGTGCCGTAA AGCTCTAAAT CGGAACCCTA AAGGGAGCCC CCGATTTAGA
7981 GCTTGACGGG GAAAGCCGGC GAACGTGGCG AGAAAGGAAG GGAAGAAAGC GAAAGGAGCG
8041 GGCGCTAGGG CGCTGGCAAG TGTAGCGGTC ACGCTGCGCG TAACCACCAC ACCCGCGCGC
8101 TTAATGCGCC GNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN
8161 NNNNNNNNNN NNNNN
//
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