pBG211 plasmid Sindbis virus辛德畢斯病毒質(zhì)粒載體 BioVector NTCC質(zhì)粒載體菌種細(xì)胞基因保藏中心
Plasmid constructs. The full-length double subgenomic Sindbis genome TE3'2J was engineered behind a cytomegalovirus promoter (CMV), and a 3' hepatitis delta virus ribozyme (HDV) was added after a 35 base adenine repeat (A35) to cleave the viral RNA from the over length transcript. pBG167 possess a unique XbaI site after the second SGP, and is analogous to pTE3'2J [17]. pBG218 is pBG167 with GFP inserted at XbaI site via ligation between the unique XbaI site in pBG167 and engineered NheI sites flanking the GFP ORF. pBG210 and pBG211 have the attR1/attR2 Gateway Recombination Cassettes ligated into the pBG167 XbaI site in forward and reverse orientation, respectively. pBG212 is the product of a LR Clonase reaction between pBG210 and pBG440 and places GFP in the sense orientation. pBG213 is a recombination between pBG211 and pBG440, and places GFP in the reverse orientation. pBG451 is the product of a recombination between pBG210 and pBG403, and places Renilla luciferase in the sense orientation. pBG452 is the product of a recombination between pBG210 and pBG344 and places mCherry in the sense orientation.
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