SARS-CoV-2 (2019-nCoV) Nucleocapsid Protein (His tag): 產(chǎn)品信息
純度> 90 % as determined by SDS-PAGE.
內(nèi)毒素< 1.0 EU per μg protein as determined by the LAL method.
生物活性Testing in progress
蛋白構(gòu)建A DNA sequence encoding the NCP-CoV(2019-nCoV) Nucleocapsid Protein was expressed with a polyhistidine tag at the C-terminus.
表達(dá)宿主Baculovirus-Insect Cells
種屬2019-nCoV
預(yù)測(cè) N 端Met
分子量The recombinant NCP-CoV(2019-nCoV) Nucleocapsid Protein (His tag) consists of 430 amino acids and predicts a molecular mass of 47.08 kDa.
緩沖液Lyophilized from sterile 20mM Tris, 500mM NaCl, pH8.0, 10%glycerol
Please contact us for any concerns or special requirements.
Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the hard copy of CoA.
運(yùn)輸方式In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.
Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
穩(wěn)定性 & 儲(chǔ)存條件Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃
Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
復(fù)溶A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
SARS-CoV-2 (2019-nCoV) Nucleocapsid Protein (His tag): 圖片
SARS-CoV-2 (2019-nCoV) Nucleocapsid Protein (His tag): 別稱
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CoV Nucleocapsid 背景信息
Coronaviruses are enveloped viruses with a positive-sense RNA genome and with a nucleocapsid of helical symmetry. Coronavirus nucleoproteins localize to the cytoplasm and the nucleolus, a subnuclear structure, in both virus-infected primary cells and in cells transfected with plasmids that express N protein. Coronavirus N protein is required for coronavirus RNA synthesis, and has RNA chaperone activity that may be involved in template switch. Nucleocapsid protein is a most abundant protein of coronavirus. During virion assembly, N protein binds to viral RNA and leads to formation of the helical nucleocapsid. Nucleocapsid protein is a highly immunogenic phosphoprotein also implicated in viral genome replication and in modulating cell signaling pathways. Because of the conservation of N protein sequence and its strong immunogenicity, the N protein of coronavirus is chosen as a diagnostic tool.
研究領(lǐng)域
Microbiology>>Pathogenic microorganism>>viruses>>Animal virus>>RNA virus>>+ssRNA virus>>Coronaviridae>>Coronavirus (CoV)>>CoV viral antigen
參考文獻(xiàn)
1.Van Boheemen S, et al. (2012), MBio. 3(6):e00473-12.
Bisht H. et al., 2004, Proc Natl Acad Sci. 101 (17): 6641-6.
Li W. et al., 2005, Science. 309 (5742): 1864-8.

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