popt_mvenus_paro plasmid vector質粒載體 BioVector NTCC質粒載體菌種細胞基因保藏中心
Vector: pBluescript backbone, pOptimized synthetic Chlamydomonas expression cassettes

Origin: synthetic construct (DNA synthesis and cloning)

Insert: pOptimized template vector containing the mVenus (YFP variant) reporter expression cassette and an antibiotic selection marker expression cassette. Both cassettes contain the HSP70A-RBCS2-InI promoter and RBCS2 3’UTR surrounding either the reporter or the marker. Each reporter contains intron 2 of RBCS2 within its nuclear sequence. Selection marker for Chlamydomonas is paromomycin resistance aphVIII (Sizova et al. 2001).

Host strain: DH5α

Selection: Ampicillin resistance for E. coli culture
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