pET28a/Cas9-Cys原核表達(dá)Cas9質(zhì)粒載體 BioVector NTCC質(zhì)粒載體菌種細(xì)胞基因保藏中心
Expresses N-terminal His tag fused human codon-optimized Cas9 nuclease having C-terminal Cysteine
A sequence encoding Cas9 with a cysteine at the C-terminus was prepared by PCRamplification using a previously described Cas9 plasmid (Cho et al, Nat Biotechnol. 2013)(Addgene: 43945) as the template and then cloned into the pET28-(a) vector (Novagen, Merk Millipore, Germany), whichincludes a sequence encoding a His-tag at the N-terminus.
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