pFD288 plasmid vector質(zhì)粒載體 BioVector NTCC質(zhì)粒載體菌種細胞基因保藏中心
The effect of the DNA source on transformation was tested using the shuttle vector pFD288. Plasmid DNA isolated fromBacteroides uniformis, Bacteroides ovatus, orBacteroides thetaiotaomicron transformedB. fragilis 638 at frequencies 7.5- to 12.5-fold less than those observed for controls with homologous DNA. Further reductions were seen withEscherichia coli purified pFD288, which transformed at 1000-fold lower frequencies. Finally, using homologous pFD288 or pBI191 isolated from strain 638, several strains ofB. fragilis, B. uniformis, andB. ovatus were transformed successfully without modification of the standard assay system.
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