NCI-H23 cell line細(xì)胞株細(xì)胞系 BioVector NTCC質(zhì)粒載體菌種細(xì)胞基因保藏中心
Organism Homo sapiens, human
Tissue lung
Product Format frozen
Morphology epithelial
Culture Properties adherent
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Disease adenocarcinoma; non-small cell lung cancer
Age 51 years
Gender male
Ethnicity Black
Derivation
This line was derived from a lung cancer obtained from a patient prior to therapy.


Clinical Data
51 years
Black
male
This line was derived from a lung cancer obtained from a patient prior to therapy.
Oncogene myc +; src +; abl +; erb +; ras +; sis -
Genes Expressed
myc +; src +; abl +; erb +; ras +; sis -
Comments
The cells carry the K-ras 12 mutation, and there is a mutation in codon 246 (ATC -> ATG, isoleucine -> methionine) of the p53 gene.
There is expression of C-myc, L-myc, v-src, v-abl, v-erb B, c-raf 1, Ha-ras, Ki-ras and N-ras RNAs.
The cells express heterogeneous mRNA expression for PDGF A and B chain, transforming growth factor alpha and beta and the epidermal growth factor receptor (EGFR).
NCI-H23 exhibits a high degree of c-myc DNA amplification (20-fold) but no detectable amplification of c-myc RNA.
The cells stain positive for keratins 5+8 and 18 and vimentin but are negative for neurofilament.
NCI-H23 cells are L-dopa decarboxylase-negative.
They have a reported colony forming efficiency of 9.7% in soft agarose.
STR Profile
Amelogenin: X
CSF1PO: 10
D13S317: 12
D16S539: 11
D5S818: 12,13
D7S820: 9,10
THO1: 6
TPOX: 8,9
vWA: 16,17
Population Doubling Time 38 hrs
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