HUP-T3 Cell line細(xì)胞株 BioVector NTCC質(zhì)粒載體菌種細(xì)胞基因保藏中心
HUP-T3 Cells
Cat No.: ACC259

Cell line:
HUP-T3
Cat
ACC259
Species:
human (Homo sapiens)
Cell type:
pancreas carcinoma
Origin:
established from the malignant ascites of a 66-year-old Japanese man with pancreas carcinoma in 1984
Biosafety level:
1
Permissions and restrictions:
A

Cell Culture Data:
Morphology:
epitheloid cells growing adherently in monolayers and clusters; image

Medium:
90% MEM (with Earle's salts) + 10% h.i. FBS + 1% non-essential amino acids + 1% sodium pyruvate
Subculture:
seed out about 1 x 106 cells/175 cm2; split confluent culture 1:6 to 1:10 once a week using trypsin/EDTA
Incubation:
at 37 °C with 5% CO2
Doubling time:
about 40-50 hours
Harvest:
about 14 x 106 cells/175 cm2
Storage:
frozen with 70% medium, 20% FBS, 10% DMSO

Scientific Data:
Mycoplasma:
negative in DAPI, microbiological culture, RNA hybridization assays
Immunology:
cytokeratin +, cytokeratin-7 (+), cytokeratin-8 +, cytokeratin-17 -, cytokeratin-18 +, cytokeratin-19 +, desmin -, endothel -, EpCAM +, GFAP -, neurofilament -, vimentin +
Fingerprint:
multiplex PCR of minisatellite markers revealed a unique DNA profile
Species:
confirmed as human with IEF of AST, NP
Cytogenetics:
human flat-moded hypodiploid karyotype with 13% polyploidy - 39(36-40)<2n>XY, -6, -8, -9, -10, -12, -13, -17, -17, -19, -20, +3mar (2 rings), del(4)(p15), add(11)(q22.3;HSR), add(12)(q24;HSR), add(19)(p13), add(21)(p12) - gene amplification suggested by large HSR - ch 17 nullisomy
Viruses:
ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HHV-8 -, HIV-1 -, HIV-2 -, HTLV-I/II -, MLV -

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