gWIZ β-gal BioVector NTCC質(zhì)粒載體菌種細(xì)胞基因保藏中心
gWIZ vectors represent a new series of
plasmids that have been engineered to
produce the highest levels of transgene
expression in a wide range of mammalian
cells and tissues. It contains a
proprietarily modified promoter followed
by the intron A from the human
cytomegalovirus (CMV) immediate early
gene and a high-efficiency artificial
transcription terminator. The expression
vector is constructed in the context of a
plasmid backbone extensively modified to
achieve the enhanced levels of trangene
expression in mammalian cells as well as
high efficiency of plasmid production in
E. coli.
INTRODUCTION
The CMV immediate early gene (IE) promoter/enhancer is
the most widely used constitutive promoter for expressing
high levels of trangene product in many mammalian cells
and tissues. However, not all CMV IE gene
promoter/enhancer-based expression vectors are created
equal. Depending on the actual CMV IE gene sequences
used and the context of the plasmid backbone upon which
the expression cassette is constructed, the expression levels
can vary as much as two orders of magnitude. The CMV IE
promoter sequences contained in the gWIZ vectors have
been systemically analyzed and modified. The
modifications include removing the sequences that are
redundant and deleterious to the high levels of expression
while retaining those sequences that are of high
transcriptional potency. After coupling the modified
promoter with a high-efficiency synthetic transcriptional
terminator, the whole expression cassette is finally
constructed on a plasmid backbone that has also been
streamlined and modified to accommodate the high levels of
expression in mammalian cells as well as high yield of
plasmid production in E. coli. The resulting plasmid, gWIZ
expression vector, is capable of fully unleashing the
potential of the CMV promoter and giving the highest levels
of expression possible both in vitro and in vivo.
USAGE
? For extremely high levels of transgene expression in
mammalian cells and tissues
【Supplier來源】BioVector NTCC Inc.
TEL：+86-010-53513060
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