pCC2FOS BioVector NTCC質(zhì)粒載體菌種細胞基因保藏中心

pCC2FOS文庫構(gòu)建Fosmid

The CopyControl Cloning System,* based on technology developed by Dr. WaclawSzybalski 1-3 at the University of Wisconsin-Madison, combines the clone stabilityafforded by single-copy cloning with the advantages of high yields of DNA obtained by“on-demand” induction of the clones to high-copy number. For example, CopyControlBAC (Bacterial Artificial Chromosome) clones can be induced to 10-20 copies per celland CopyControl Fosmid and PCR clones can be induced from single-copy to 10-200copies per cell to improve DNA yields for sequencing, fingerprinting, subcloning, in vitrotranscription, and other applications. CopyControl Cloning Kits for BAC cloning, fosmidlibrary production, and cDNA, Gene & PCR cloning are available.The CopyControl Cloning System Has Two Required Components1. Each CopyControl Vector contains both a single-copy origin and the high-copy oriVorigin of replication. Initiation of replication from oriV requires the trfA gene productthat is supplied by the second system component, the EPI300?-T1R E. coli strain.*2. The EPI300 E. coli provides a mutant trfA gene whose gene product is required forinitiation of replication from oriV. The cells have been engineered so that the trfAgene is under tight, regulated control of an inducible promoter. Phage T1-resistantEPI300-T1R cells are provided with the kits.

Features of the CopyControl pCC1FOS? and pCC2FOS? Vectors? Chloramphenicol resistance as an antibiotic selectable marker.? E. coli F factor-based partitioning and single-copy origin of replication.? oriV high-copy origin of replication.? Bacteriophage lambda cos site for lambda packaging or lambda-terminase cleavage.? Bacteriophage P1 loxP site for Cre-recombinase cleavage.? Bacteriophage T7 RNA polymerase promoter flanking the cloning site.

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