pPR3-N Information
Function yeast Hybrid plasmid

Promoter: CYC1 promoter

Replicator: pUC ori, F1 ori, 2 mu ori

Terminator: CYC1 Terminator

Plasmid classification: yeast cell plasmids, yeast hybrid plasmids, two hybrid plasmids.

Plasmid size: 6200bp

Plasmid label: N-NubG; N-HA

Prokaryotic resistance: ampicillin Amp

Screening markers: TRP1

Cloned strains of Escherichia coli, DH5 A and other Escherichia coli

Culture conditions: 37 centigrade, LB, aerobic

Expression host: yeast

Culture conditions: 30 centigrade, YPD, aerobic

5'sequencing primers: CYC1-F:CTTTCCTTATACATTAGGACC

3'sequencing primers: CYC1-R:GGGACCTAGACTTCAGGTTG

Note: high copy plasmids

pPR3-N Description

PPR3-N is a plasmid for yeast two hybrid experiment of membrane proteins.The DUALmembrane system is intended for the detection and identification of interactions involving integral membrane proteins and membrane-associated proteins in yeast. The DUALmembrane pairwise interaction kit enables you to: Investigate the interaction between a membrane protein (integral or membrane-associated) and a membrane protein or soluble protein Map domains or amino acids which are critical for an interaction Screen cDNA libraries using a membrane protein as a bait to find novel interacting prot

eins (requires purchase of a separate cDNA library from Dualsystems)