pOSEL144乳酸菌表達(dá)載體 BioVector NTCC質(zhì)粒載體菌種細(xì)胞基因保藏中心

The pOSEL144 is a parental construct to pOSEL175, except the latter had two MfeI sites within the erm gene removed. Schematic of the pOSEL144 expression vector. The repA and erm genes are derived from a naturally occurring L. reuteri plasmid pLEM7 (14) and the ColE1 replicon is from pBluescript. An expression cassette comprising the P23 promoter from Lactococcus lactis, the CbsAss from L. crispatus, and the 2D CD4 coding region was inserted into SacI and XbaI sites of pOSEL144. The resulting plasmid was designated as pOSEL651.
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