E11 Fish  Fibroblast cell line魚類成纖維細(xì)胞株 BioVector NTCC質(zhì)粒載體菌種細(xì)胞基因保藏中心
E11 Fish  Fibroblast cell line
Supplied by: European Collection of Authenticated Cell Cultures (ECACC)
Culture Type: Cell line
Collection: ECACC General Collection
Catalogue No.: 01110916
Cell Line Name: E11
Citation Guidance: If use of this culture results in a scientific publication, it should be cited in the publication as: E11 (ECACC 01110916)
Keywords: Snakehead fish, whole fry tissue
Cell Line Description: E11 is a clone of the cell line SSN-1 and is persistantly infected with a C-type retrovirus (SnRV). It is susceptible to piscine nodavirus strains belonging to different genotypes (SJNNV, RGNNV, TPNNV and BFNNV - striped jack, red spotted grouper, tiger puffer and barfin flounder nervous necosis viruses repectively). E11 is highly permissive to nodavirus infection and production. An advantage of E11, over the parental line SSN-1, is its steady, faster growth to confluency and longer stability in monolayer cultures for 2 weeks at 25°C or 4 weeks at 20°C and its reproducible, clear CPE characterised by cytoplasmic vacuole formation followed by intensive disintegration.
Species: Fish
Tissue of Origin: Fish, whole
CellType: Fibroblast
Growth Mode: Adherent
Karyotype: Not specified
Biosafety Information: Unless specified otherwise, at the European Collection of Authenticated Cell Cultures (ECACC) we routinely handle all of our cell lines at containment level 2 in accordance with the ACDP guidelines. ACDP = Advisory Committee on Dangerous Pathogens (UK)
All cell cultures have the potential to carry as yet unidentified adventitious agents. It is the responsibility of the end user to ensure that their facilities comply with biosafety regulations for their own country.

ACDP Guidance: Biological agents: Managing the risks in laboratories and healthcare premises.

Hyperlinks to MSDS documents:
Frozen cell cultures Material Safety Data Sheet
Growing cell cultures Material Safety Data Sheet
Nucleic acids derived from cell cultures Material Safety Data Sheet
Subculture Routine: Split sub-confluent cultures (70-80%) 1:4 ie. seeding at 3 x10,000 cells/cm2 using 0.25% trypsin/EDTA. No CO2. Culture at 25°C
Culture Medium: L15 + 5% Foetal Bovine Serum (FBS)+ 2mM Glutamine
Depositor: Professor Toshihiro Nakai, PhD Laboratory of Fish Pathology Graduate School of Biosphere Science Hiroshima University 1-4-4 Kagamiyama, Higashi-Hiroshima Hiroshima 739-8528, Japan
Originator: Yes
Country: Japan
References: Iwamoto et al (2000), Cloning of the Fish Cell Line SSN-1 for Piscine Nodaviruses In : Diseases of Aquatic Organisms,43 p81-89
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