pEXP-AD502 BioVector NTCC質(zhì)粒載體菌種細(xì)胞基因保藏中心

pEXP-AD502酵母雙雜交載體，帶有ADH啟動(dòng)子和終止子，GAL4AD，Gateway技術(shù)兼容，Amp抗性。TRP1酵母篩選標(biāo)記。
pEXP-AD502 is an Activation Domain (AD) Gateway? Expression Vector. This
plasmid is used to construct a cDNA or genomic library for identifying proteins
(AD-Y) that interact with the fusion protein (DB-X).

Features of this vector include:
? the sequence encoding the GAL4 Activation Domain (amino acid 768-881)
fused to the nuclear localization signal from SV40 Large T antigen,
? the ARS4/CEN6 sequence for replication and low-copy-number maintenance
in yeast,
? the TRP1 gene for selection in yeast on medium lacking tryptophan,
? the constitutive moderate-strength promoter and transcription terminator of
the yeast Alcohol Dehydrogenase gene (ADH1) expressing the GAL4 AD,
? a pUC-based replication origin and ampicillin resistance gene (Apr
) for
replication and maintenance in E. coli, and
? two recombination sites, attB1 and attB2, flanking a multiple cloning site,
including Sal I and Not I sites for generation of cDNA libraries using the
SuperScript? Plasmid System with Gateway? Technology for cDNA
Synthesis and Plasmid Cloning.
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