• Vector backbone
  pValium22
  (Search Vector Database)
• Backbone manufacturer
  Norbert Perrimon, Jian-Quan Ni, Harvard

• Total vector size (bp)6227

• Vector type
  Insect Expression, CRISPR

GROWTH IN BACTERIA

• Bacterial Resistance(s)
  Ampicillin
• Growth Temperature
  37°C
• Growth Strain(s)
  DH5alpha
• Copy number
  Unknown

GENE/INSERT

• Gene/Insert name
  dU6-2:gRNA
• Species
  D. melanogaster (fly)
• Insert Size (bp)
  730

• PromoterdU6-2

CLONING INFORMATION

• Cloning methodLigation Independent Cloning

• 5′ sequencing primerCAAACTCTCTTAGTCGTGCC

• 3′ sequencing primerTGCATACGCATTAAGCGAAC