pCas 大腸桿菌基因編輯質(zhì)粒RED重組Cas9載體

Bacterial Expression, CRISPR ; Lambda Red recombinasenative cas9 promotercas9 (Other)

Constitutive expression of cas9 and inducible expression of lambda RED and sgR

Vector backbonepKD46

Backbone size w/o insert (bp)6300

Modifications to backboneReplace Amp with Kan

Vector typeBacterial Expression, CRISPR ; Lambda Red recombinase

GROWTH IN BACTERIA

Bacterial Resistance(s)Kanamycin

Growth Temperature30°C

Growth Strain(s)DH5alpha

Growth instructionsMust be grown at 30C! Vector has temperature-sensitive replication (RepA101ts)

Copy numberLow Copy

GENE/INSERT

Gene/Insert namecas9

SpeciesS. pyogenes MGAS5005

Insert Size (bp)4500

Promoternative cas9 promoter

BioVector NTCC質(zhì)粒載體菌株細(xì)胞蛋白抗體基因保藏中心

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