Mus musculus lung macrophage cell line小鼠肺泡巨噬細胞株 BioVector NTCC質粒載體菌種細胞基因保藏中心
Mus musculus lung macrophage cell line
Cat No.: NTCC595981

Organism Mus musculus, mouse
Tissue lung
Cell Type macrophage (alveolar)
Product Format frozen
Morphology macrophage
Culture Properties mixed, adherent and suspension
Biosafety Level 2    [Cells may contain SV40 virus]

Age 7 weeks
Gender male
Derivation The MUS MUSCULUS LUNG MACROPHAGE cell line was derived by SV40 transformation of an adherent cell enriched population of mouse alveolar macrophages.
Clinical Data male
7 weeks
Antigen Expression CD11b (Mac-1); Class II antigens (I-A); T antigen
Receptor Expression Fc

Genes Expressed interleukin 1 (IL-1)
Cellular Products interleukin 1 (IL-1)
Comments The cells retain many of the properties of alveolar macrophages including typical macrophage morphology.
They are adherent, phagocytic, esterase positive and peroxidase negative.
Lipopolysaccharide (LPS) treatment stimulates IL-1 production.
The cells are capable of suppressing the in vitro plaque forming cell (PFC) response in a cell dose dependent manner.
Complete Growth Medium The base medium for this cell line is RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: 2-mercaptoethanol to a final concentration of 0.05 mM; fetal bovine serum to a final concentration of 10%.
Subculturing Cultures can be maintained by transferring floating cells to a centrifuge tube Rinse adherent cells with 0.25% trypsin, 0.53 mM EDTA solution. Remove the solution and add an additional 1 to 2 mL of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37°C) until the cells detach. Add to floating cells collected above and centrifuge the cell suspension at 1000 rpm for 10 minutes, resuspend the pellet in fresh medium, aspirate and dispense into new flasks.
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended
Medium Renewal: Every 2 to 3 days
Culture Conditions Temperature: 37°C
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