ATCC Medium: 260 Tryptic Soy Agar/Broth with 5% Sheep Blood (defibrinated) BioVector NTCC質(zhì)粒載體菌種細胞基因保藏中心
ATCC Medium: 260 Tryptic Soy Agar/Broth with 5% Sheep Blood (defibrinated)
Agar Medium Tryptic Soy Agar (BD 236950)……………40 g
Sheep Blood (defibrinated)………………..50 ml
DI Water……………………………………...950 ml
Autoclave at 121oC. Cool sterilized medium to ~47oC.
Aseptically add 50 ml of room temperature defibrinated sheep blood. Gently mix and dispense as required.
Broth Medium
Tryptic Soy Broth (BD 211825)…………….30.0 g
Sheep Blood (defibrinated)…………………50 ml
DI Water…………………………………..…..950 ml
Follow same procedure as agar medium above. If commercialized product is not available, Tryptic Soy Agar can be made from scratch, by following the formulation below:
Tryptic Soy Agar Composition
Tryptone…………………………15.0 g
Soytone………………………….5.0 g
NaCl……………………………...5.0 g
Agar……………………………...15.0 g
DI Water………………………….950 ml
pH to 7.3 +/- 0.2.
(Tryptone-Pancreatic Digest of Casein; Soytone-Papaic Digest of Soybean Meal). To make Tryptic Soy Broth from scratch, follow Tryptic Soy Agar formulation but omit agar.
【Supplier來源】BioVector NTCC Inc.
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