pCC1 BAC BioVector NTCC質(zhì)粒載體菌種細(xì)胞基因保藏中心

pCC1BAC拷貝控制型BAC克隆質(zhì)粒，Cm氯霉素抗性，F(xiàn)-factor單拷貝復(fù)制子和誘導(dǎo)型oriV多拷貝復(fù)制子，P1噬菌體loxP位點(diǎn)用于Cre重組酶切割。
The CopyControl? pCC1BAC? Vector* is based on an innovative technology originally
developed in the laboratory of Dr. Waclaw Szybalski1
and optimized at Epicentre.2
The vector has two origins of replication – a single-copy E. coli F-factor replicon and a
high-copy origin of replication called “oriV”. Initially, replication of CopyControl clones
can be controlled by the F-factor replicon so the vector is present at one copy per cell.
Maintaining clones at single copy ensures insert stability and allows cloning of toxic gene
products (Fig. 1).
Initiation of replication from oriV requires the trfA gene product. CopyControl Vectors use
a specifically engineered E. coli host strain, TransforMax? EPI300? (available separately),
which contains a mutant trfA gene under tight control of an inducible promoter. Addition
of the CopyControl Induction Solution to the growth medium induces expression of
trfA and subsequent amplification of the clone to high-copy number. Induction of
CopyControl BAC clones from single-copy up to 10-20 copies per cell greatly improves
the yield and purity of BAC DNA for sequencing, fingerprinting and other applications.
The CopyControl pCC1BAC Vector is derived from pBeloBAC113 and Epicentre’s
pIndigoBAC-5. The vector has been linearized at a unique restriction enzyme recognition
site (BamH I, Hind III or EcoR I), dephosphorylated, and highly purified to ensure very low
background. Features of the vector include:
?  Chloramphenicol-resistance as an antibiotic selectable marker.
?  E. coli F factor-based partitioning and single-copy number regulation system.
?  oriV high-copy origin of replication.
?  Primer binding sites for BAC-end sequencing
?  Not I sites surrounding the BamH I, Hind III and EcoR I cloning sites.
?  Bacteriophage P1 loxP site for Cre-recombinase cleavage.
【Supplier來(lái)源】BioVector NTCC Inc.
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