JM109(DE3) E.coli strain大腸桿菌菌株 BioVector NTCC質(zhì)粒載體菌種細(xì)胞基因保藏中心
Bacterial Strain JM109(DE3), derived from JM109, contains a chromosomal copy of the gene for T7 RNA polymerase. JM109(DE3) is used for the high-level expression of genes cloned into vectors for expression of sequence downstream from the T7 promoter, provided that the cloned sequence contains a ribosomal binding site. Please note: JM109(DE3) cannot be used for blue/white selection.
Genotype: endA1, recA1, gyrA96, thi, hsdR17 (rk–, mk+), relA1, supE44, λ–, Δ(lac-proAB), [F′, traD36, proAB, lacIqZΔM15], lDE3.
For High-Level Gene Expression from the T7 Promoter
? Contains an IPTG-inducible gene for T7 RNA polymerase
? Requires a ribosomal binding site with cloned sequence
? Lacks blue/white cloning selection


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