pREDTKI大腸桿菌基因重組載體

BioVector NTCC質(zhì)粒載體菌種細(xì)胞基因保藏中心

pREDTKI大腸桿菌基因重組載體
E coli Genetic Modification
Vector backbone pKD46
(Search Vector Database)
Backbone size w/o insert (bp) 6300
Modifications to backbone replace amp to kan
Vector type Bacterial Expression ; Red recombinase and I-SceI endonuclease
GROWTH IN BACTERIA

Bacterial Resistance(s) low Kan
Growth Temperature 30°C
Growth Strain(s) DH5alpha
Growth instructions Culture the plate in 30 C or 28 C for 24h or up to 48h to see if any colonies appear. LB +1% glucose plate, or SOC plate improve growth.
Copy number Low Copy
GENE/INSERT

Gene/Insert name I-SceI
Alt name mitochondrial intron of Saccharomyces cerevisiae
Species S. cerevisiae (budding yeast)
Insert Size (bp) 3000
Entrez Gene SCEI (a.k.a. Q0160)
Promoter trc
CLONING INFORMATION

Cloning method Restriction Enzyme
5′ cloning site NcoI (not destroyed)
3′ cloning site NcoI (not destroyed)
5′ sequencing primer none
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