DH10B T1R E.coli大腸桿菌菌株、感受態(tài)細(xì)胞 BioVector NTCC質(zhì)粒載體菌種細(xì)胞基因保藏中心
DH10B T1 E.coli DH10B T1,1vial, Storage:4℃
Features of DH10B T1 Phage-Resistant strain:
? Permit efficient cloning of methylated DNA
? Are resistant to T1 and T5 bacteriophages
? Support blue/white screening by α-complementation on plates containing X-Gal or Bluo-Gal
? Deliver high-yield plasmid preparations for downstream applications
Phage-resistant representative libraries from a single transformation
The tonA mutation in DH10B cells confers resistance to bacteriophages T1 and T5, safeguarding valuable
libraries against contamination. Additionally, mutations in the methylation-dependent restriction system
(mcrA, mcrBC, and mrr) make DH10B T1 Phage-Resistant Cells ideal for construction of genomic libraries of
both prokaryotic and eukaryotic genomic DNA, and allow for efficient plasmid rescue from eukaryotic genomes.
DH10B T1 Phage-Resistant Cells are suitable for construction of gene banks, for the generation of cDNA
libraries using plasmid-derived vectors, and for situations when DNA is limiting. This strain also has the
Φ80lacZΔM15 genotype, providing for the option of blue/white screening on plates containing either X-Gal or
Bluo-Gal. Finally, theendA1 mutation makes DH10B an excellent vehicle for amplifying plasmid DNA for
subsequent extraction and purification.
Genotype: F-mcrA Δ(mrr-hsdRMS-mcrBC) Φ80lacZΔM15
ΔlacX74 recA1 endA1araD139Δ(ara, leu)7697 galU galK λ-rpsL nupG tonA
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