pFungi-Cas9-hygro真菌基因編輯CRISPR-Cas9載體

BioVector NTCC質(zhì)粒載體菌種細(xì)胞基因保藏中心                      

Description
AMA1 plasmid with Aspergillus optimized Cas9 and hph selection marker.
Total vector size (bp) 15567
Vector type Bacterial Expression, CRISPR ; Fungal expression
Selectable markers Hygromycin
Bacterial Resistance(s) Ampicillin
Growth Temperature 37°C
Growth Strain(s) DH5alpha
Gene/Insert name Cas9
Species Synthetic; synthetic gene, codon optimized for A. niger
Insert Size (bp) 4131  GenBank ID KT031983
Promoter: Aspergillus nidulans tef1 promoter
Tag / Fusion Protein: SV40 NLS (C terminal on insert)
5′ sequencing primer: CTTCTCTGCTCAGCACCTCTACG
Gene/Insert name hph (hygromycin resistance marker)
Insert Size (bp) 1026
Promoter: Aspergillus nidulans trpC promoter
5′ sequencing primer: GCTAGTGGAGGTCAACACATCAATGC

BioVector NTCC質(zhì)粒載體菌種細(xì)胞蛋白抗體基因保藏中心
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