1321N1 cells細(xì)胞株

BioVector NTCC質(zhì)粒載體菌種細(xì)胞基因保藏中心                      

Cell Line Name: 1321N1

Citation Guidance: If use of this culture results in a scientific publication, it should be cited in the publication as: 1321N1 (ECACC 86030402)
Keywords: Human brain astrocytoma
Cell Line Description: 1321N1 is a human astrocytoma cell line isolated in 1972 as a sub clone of the cell line 1181N1 which in turn was isolated from the parent line U-118 MG (one of a number of cell lines derived from malignant gliomas by J Ponten (Pontén, J., Macintyre, E. H. (1968) PMID: 4313504).1321N1 has been shown to have very similar STR profile data to U-118 MG.
Species: Human
Tissue of Origin: brain
CellType: Glial
Growth Mode: Adherent
DNA Profile: STR-PCR Data:
Amelogenin: X
CSF1PO: 11,12
D13S317: 9,11
D16S539: 12,13
D5S818: 11
D7S820: 9
THO1: 6
TPOX: 8
vWA: 18

Karyotype: Not specified
Biosafety Information: Unless specified otherwise, at the European Collection of Authenticated Cell Cultures (ECACC) we routinely handle all of our cell lines at containment level 2 in accordance with the ACDP guidelines. ACDP = Advisory Committee on Dangerous Pathogens (UK)
All cell cultures have the potential to carry as yet unidentified adventitious agents. It is the responsibility of the end user to ensure that their facilities comply with biosafety regulations for their own country.

ACDP Guidance: Biological agents: Managing the risks in laboratories and healthcare premises.

Hyperlinks to MSDS documents:
Frozen cell cultures Material Safety Data Sheet
Growing cell cultures Material Safety Data Sheet
Nucleic acids derived from cell cultures Material Safety Data Sheet
Subculture Routine: Split sub-confluent cultures (70-80%) 1:2 to 1:6 i.e. seeding at 2-4x10,000 cells/cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37°C.
Culture Medium: DMEM + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).
Depositor: Dr M Shaw, ICI Pharmaceuticals Ltd
Originator: No
Country: UK
References: Macintyre EH, Pontén J, Vatter AE. 1972 The ultrastructure of human and murine astrocytes and of human fibroblasts in culture. Acta Pathol Microbiol Scand A. 80(2):267-83 PMID: 4337530. Macintyre EH, Wintersgill CJ, Thormar H 1972 The establishment of a line of visna virus-producing human astrocytes (V-1181N1).Med Res Eng. 11(4):7-13 PMID: 4370460.
Additional Bibliography: Foster SJ, Perkins JP. 1977 Glucocorticoids increase the responsiveness of cells in culture to prostaglandin E1.Proc Natl Acad Sci U S A. 74(11):4816-20 PMID: 200933. Barretina J, et al., 2012 The Cancer Cell Line Encyclopedia enables predictive modelling of anticancer drug sensitivity. Nature. 483(7391):603-7. PMID: 22460905.

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