pMonAID_nCas9-PmCDA_Hyg_ALS  BioVector NTCC質(zhì)粒載體菌種細(xì)胞基因保藏中心                      

Monocot Target-AID vector expressing rice-optimized nCas9-PmCDA1 with sgRNA targeting OsAls
Vector backbone pZDgRNA_Cas9ver.2 (D10A)_HPT
(Search Vector Database)
Backbone size w/o insert (bp) 17773
Vector type Plant Expression
Selectable markers Hygromycin
GROWTH IN BACTERIA

Bacterial Resistance(s) Spectinomycin
Growth Temperature 37°C
Growth Strain(s) DH5alpha
Copy number Unknown
GENE/INSERT

Gene/Insert name SpCas9
Species Streptococcus pyogenes
Mutation D10A for nickase Cas9
Promoter 2x35S
Tag / Fusion Protein
PmCDA1 (C terminal on insert)
CLONING INFORMATION

Cloning method Gibson Cloning
5′ sequencing primer ggaagttcatttcatttggagag
3′ sequencing primer ccatttgcattttgatgtccg

BioVector NTCC質(zhì)粒載體菌種細(xì)胞蛋白抗體基因保藏中心
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