WDC-1 cell line

Duodenalcarcinoma is very rare and its culture cell lines have rarely been established.

Tumor cells separated from a surgically resected primary tumor of duodenal carcinoma were put into culture. The patient was an 81-year-old female and had metastatic lymph nodes.

A new cell line, designated WDC-1, was established. This duodenal carcinoma cell line proliferated in a monolayered sheet with a doubling time of 50 h. The histological findings of the xenograft in nude mice were similar to those of the primary tumor. WDC-1 cells produced carcinoembryonic antigen and expressed 1 integrin and very late antigen (VLA)-4d in vitro.

Culture medium:

RPMI1640 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS) + 100 U/ml penicillin G sodium (option); 100 μg/ml streptomycin sulfate (option);

<br/>BioVector NTCC質(zhì)粒載體菌種細(xì)胞蛋白抗體基因保藏中心

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