C0134人B淋巴母細胞B-lymphoblastoid cells BioVector NTCC保藏中心
Gender性別:Male
Age at venesection采血時年齡：48

【Organism物種來源】Human人源
【Tissue組織來源】
【Cell Type細胞類型】
【Product Format產(chǎn)品狀態(tài)】 frozen/live culture
【Morphology細胞形態(tài)】
【Culture Properties細胞特性】
【Biosafety Level生物安全級別】 1/2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

【Disease細胞源疾病】
【Age年齡】
【Gender性別】 Male/Female
【Storage Conditions保存條件】 liquid nitrogen vapor phase
【Karyotype染色體組型】
【Images細胞圖片】 Cell Micrograph
【Derivation衍生細胞】 -
【Clinical Data臨床數(shù)據(jù)】
【Comments其他描述】
【Complete Growth Medium完全培養(yǎng)基】 The base medium for this cell line is DMEM/RPMI-1640 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
【Subculturing傳代方法】
Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.
Remove and discard culture medium.
Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.
Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.
Add appropriate aliquots of the cell suspension to new culture vessels.
Incubate cultures at 37°C.
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended
Medium Renewal: 2 to 3 times per week
【Cryopreservation凍存條件】
【Freeze Medium凍存培養(yǎng)基】Complete growth medium supplemented with 5% (v/v) DMSO
【Storage Temperature保存溫度】 Liquid nitrogen vapor phase
【Culture Conditions培養(yǎng)條件】
【Atmosphere培養(yǎng)環(huán)境】 Air, 95%; carbon dioxide (CO2), 5%
【Temperature培養(yǎng)溫度】 37°C
【STR Profile圖譜】
【Population Doubling Time倍增殖時間】
【References參考文獻】

【Supplier細胞供應(yīng)廠家】BioVector NTCC Inc.
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