BACKBONE

• Vector backbone
  pEGFP-C1
  (Search Vector Database)
• Backbone manufacturer
  Clontech

• Backbone size w/o insert (bp)4700

• Vector type
  Mammalian Expression
• Selectable markers
  Neomycin

GROWTH IN BACTERIA

• Bacterial Resistance(s)
  Kanamycin
• Growth Temperature
  37°C
• Growth Strain(s)
  DH5alpha
• Copy number
  Low Copy

SEQUENCE INFORMATION

• Depositor Sequences
  None.
• Addgene Sequences
  Partial (1)

Full plasmid sequence is available only if provided by the depositing laboratory.

GENE/INSERT

• Gene/Insert name
  p65
• Alt name
  GFP-p65
• Species
  H. sapiens (human)
• Insert Size (bp)
  1600
• Entrez Gene
  RELA(a.k.a. NFKB3, p65)

• Tag / Fusion Protein

  • EGFP (N terminal on insert)

  
  

CLONING INFORMATION

• Cloning methodRestriction Enzyme

• 5′ cloning siteBamHI (not destroyed)

• 3′ cloning siteXbaI (not destroyed)

• 5′ sequencing primerEGFP-C

• 3′ sequencing primerSV40pA-R

(Common Sequencing Primers)