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Acinetobacter baumannii鮑曼不動桿菌NTCC? 17-ZW_S35.2-1 Strain菌株/培養(yǎng)基/基因組DNA NTCC典型培養(yǎng)物保藏中心DSM 117601

Acinetobacter baumannii鮑曼不動桿菌NTCC? 17-ZW_S35.2-1 Strain菌株/培養(yǎng)基/基因組DNA NTCC典型培養(yǎng)物保藏中心DSM 117601

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Acinetobacter baumannii鮑曼不動桿菌NTCC? U17-HoPe-P1-1 Strain菌株/培養(yǎng)基/基因組DNA NTCC典型培養(yǎng)物保藏中心DSM 117603

Acinetobacter baumannii鮑曼不動桿菌NTCC? U17-HoPe-P1-1 Strain菌株/培養(yǎng)基/基因組DNA NTCC典型培養(yǎng)物保藏中心DSM 117603

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Acinetobacter baumannii鮑曼不動桿菌NTCC? U17-HoPeS16.1 Strain菌株/培養(yǎng)基/基因組DNA NTCC典型培養(yǎng)物保藏中心DSM 117605

Acinetobacter baumannii鮑曼不動桿菌NTCC? U17-HoPeS16.1 Strain菌株/培養(yǎng)基/基因組DNA NTCC典型培養(yǎng)物保藏中心DSM 117605

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Acinetobacter baumannii鮑曼不動桿菌NTCC? U18-HoPe-S14.1 Strain菌株/培養(yǎng)基/基因組DNA NTCC典型培養(yǎng)物保藏中心DSM 117607

Acinetobacter baumannii鮑曼不動桿菌NTCC? U18-HoPe-S14.1 Strain菌株/培養(yǎng)基/基因組DNA NTCC典型培養(yǎng)物保藏中心DSM 117607

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Acinetobacter baumannii鮑曼不動桿菌NTCC? U19-StiWa-S4-1 Strain菌株/培養(yǎng)基/基因組DNA NTCC典型培養(yǎng)物保藏中心DSM 117609

Acinetobacter baumannii鮑曼不動桿菌NTCC? U19-StiWa-S4-1 Strain菌株/培養(yǎng)基/基因組DNA NTCC典型培養(yǎng)物保藏中心DSM 117609

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Staphylococcus epidermidis表皮葡萄球菌NTCC? v11608 Strain菌株/培養(yǎng)基/基因組DNA NTCC典型培養(yǎng)物保藏中心DSM 117682

Staphylococcus epidermidis表皮葡萄球菌NTCC? v11608 Strain菌株/培養(yǎng)基/基因組DNA NTCC典型培養(yǎng)物保藏中心DSM 117682

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Enterococcus faecalis糞腸球菌NTCC? HUN086 (68A) Strain菌株/培養(yǎng)基/基因組DNA NTCC典型培養(yǎng)物保藏中心DSM 118056

Enterococcus faecalis糞腸球菌NTCC? HUN086 (68A) Strain菌株/培養(yǎng)基/基因組DNA NTCC典型培養(yǎng)物保藏中心DSM 118056

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TK2420大腸桿菌突變株NTCC? E.coli缺乏Kdp， Trk和Kup K+吸收系統(tǒng)-BioVector NTCC質(zhì)粒載體菌株細(xì)胞蛋白抗體基因保藏中心

TK2420 is a mutant strain of Escherichia coli that is deficient in the Kdp, Trk, and Kup K+ uptake systems: K+ requirement TK2420 requires exogenous K+ to maintain steady-state pH homeostasis and to recover from sudden acid shifts. Complementation The recombinant plasmid pGerN, which expresses the endospore germination protein GerN, can complement the phenotype of TK2420. This suggests that GerN may catalyze both Na+ extrusion and K+ uptake. Growth inhibition Aa-KefB and Aa-NhaP inhibit the growth of TK2420, suggesting that they catalyze K+ efflux. K+ uptake K+ uptake by TK2420 yggS-T/pUT is slightly decreased by the addition of 5mM NaCl. Osmolarity The K+ requirement of TK2420 is partly compensated by supplementation with NaCl, choline chloride, proline, or sucrose. This suggests that the K+ requirement is mediated in part by osmolarity

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E. coli K-12 MG1655 NTCC?大腸桿菌專利菌株-BioVector NTCC質(zhì)粒載體菌株細(xì)胞蛋白抗體基因保藏中心

E. coli K-12 MG1655 Description Genotype: F- lambda- ilvG- rfb-50 rph-1 Serotype: OR:H48:K- This strain was sequenced by the Blattner laboratory because it approximates wild-type E. coli and "has been maintained as a laboratory strain with minimal genetic manipulation, having only been cured of the temperate bacteriophage lambda and F plasmid by means of ultraviolet light and acridine orange, respectively." [1]. The mutations listed in the genotype are present in most K-12 strains and were probably acquired early in the history of the laboratory strain. A frameshift at the end of rph results in decreased pyrE expression and a mild pyrimidine starvation, such that the strain grows 10 to 15% more slowly in pyrimidine-free medium than in medium containing uracil [2]. The ilvG- mutation is a frameshift that knocks out acetohydroxy acid synthase II [3]. The rfb-50 mutation is an IS5 insertion that results in the absence of O-antigen synthesis [4]. MG1655 was derived and named by Mark Guyer from strain W1485, which was derived in Joshua Lederberg's lab from a stab-culture descendant of the original K-12 isolate. This original E. coli strain K-12 was obtained from a stool sample of a diphtheria patient in Palo Alto, CA in 1922 [5]. References: Blattner, et al (1997) The complete genome sequence of Escherichia coli K-12. Science 277(5331), 1453-1462. PMID: 9278503 Jensen (1993) The Escherichia coli K-12 "wild types" W3110 and MG1655 have an rph frameshift mutation that leads to pyrimidine starvation due to low pyrE expression levels. J Bacteriol 175(11), 3401-3407. PMID: 8501045 Lawther, et al. (1982) DNA sequence fine-structure analysis of ilvG (IlvG+) mutations of Escherichia coli K-12. J Bacteriol 149(1), 294-298. PMID: 7033211 Liu and Reeves (1994) Escherichia coli K12 regains its O antigen. Microbiology 140(Pt 1), 49-57. PMID: 7512872 Bachmann (1996) Derivations and Genotypes of Some Mutant Derivatives of Escherichia coli K-12. p2460-2488 in Neidhardt (ed), Escherichia coli and Salmonella, 2nd Edition ASM Press, Washington DC Media and growth curves MG1655 grows on LB and M9 minimal medium (+ Glucose + 1ug/ml thiamine). In doing experiments with microarrays, we sought a medium that was both defined and reproducible (unlike LB), yet supported fast growth rates. We now use Neidhardt's MOPS-based rich defined medium (MOPS-RDM). We also sought a commercially available rich defined medium, and finding none tried to grow MG1655 on a medium sold for mammalian cell culture. It grows quite well, but we decided to stick with Neidhardt's medium because it shows a sharper transition to stationary phase. Recipe for MOPS Rich Defined Medium and MOPS Minimal Medium Growth curve for MG1655 on MOPS Minimal Medium Information and growth curve for a commercially available rich defined medium (MEM alpha w/o phenol red) Growth curves for MG1655 on M9 Minimal Medium

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Azotobacter chroococcum W5圓褐固氮菌菌種-可產(chǎn)生吲哚乙酸植物生長激素-BioVector NTCC質(zhì)粒載體菌株細(xì)胞蛋白抗體基因保藏中心

Azotobacter chroococcum W5圓褐固氮菌菌種-可產(chǎn)生吲哚乙酸植物生長激素BioVector NTCC質(zhì)粒載體菌株細(xì)胞蛋白抗體基因保藏中心Azotobacte...

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